Makassar Pharmaceutical Science Journal (MPSJ)

TESTING ANTIOXIDANT ACTIVITY OF ETHANOL EXTRACT OF TAMPALA BAJAKAH ROOTS (Spatholobus Littoralis Hassk) USING DPPH-FREE RADICAL REDUCTION METHOD

Wina Astri Sari, Masdiana Tahir, Muzakkir Baits — Wed, 30 Oct 2024 00:00:00 +0000

Bajakah tampala (Spatholobus littoralis Hassk.) is a plant that lives on vines in tropical areas. This plant comes from Sebuku Village, North Kalimantan. The Bajakah Tampala plant contains saponins, flavonoids and tannins. The roots of the Bajakah Tampala plant are thought to have strong antioxidant activity which is able to reduce the activity of free radicals. The aim of this research is to analyze the antioxidant activity of Bajakah Tampala roots using the DPPH reduction method using a UV Vis spectrophotometer which is based on calculating the IC50 value. The results of this study indicate that the ethanol extract of Bajakah Tampala roots has antioxidant activity against DPPH free radicals with an IC50 value of 9.206 µg/mL. which is included in the group of very strong antioxidants.

FORMULASI LOTION ANTIOKSIDAN EKSTRAK KULIT PISANG KEPOK (Musa balbsiana colla) MENGGUNAKAN EMULGATOR TWEEN 80 DAN SPAN 80

Audia Triani Olii, Mumtihanah Musyid, Besse Nurfitriani — Wed, 30 Oct 2024 00:00:00 +0000

_{Kepok banana peels contain flavonoid compounds which can be used as antioxidants. Lotion is a type of cosmetic in the emollient (softener) category which has a higher air content. Its functions include maintaining skin moisture, cleaning, preventing moisture loss or retaining active ingredients on the skin. The use of an appropriate combination of emulsifiers between Tween 80 and Span 80 in lotion preparations can form an optimal oil-in-water type emulsion. The aim of this research is to optimize the formulation of kepok banana peel extract lotion (Musa balbisiana colla) with Tween 80 and Span 80 as emulsifiers. The research results showed that the lotion formula was optimized to produce a formula using Tween 80 : Span 80 of 9% : 1%, which had a pH value of 6.4467, viscosity of 12860 cPs, adhesive power of 5.66 seconds, spreadability of 6.4 cm/ seconds, and the antioxidant activity test was 79.9249. Meanwhile, the results of stability testing of the optimum lotion formula showed good stability in homogeneity, pH, spreadability and adhesion tests. However, the viscosity test was unstable.}

FORMULASI LOTION TABIR SURYA EKSTRAK KULIT PISANG RAJA (Musa paradisiaca L.) MENGGUNAKAN KONSENTRASI TWEEN 80 DAN SPAN 80

Audia Triani Olii, Mumtihanah Mursyid, Fitriyah Maya Shinta — Thu, 31 Oct 2024 01:04:31 +0000

Plantain peel contains flavonoid compounds that can be used as sunscreen. The purpose of this research is to optimize the preparation of plantain peel extract lotion (Musa paradisiaca L.). The use of Tween 80 and Span 80 in lotion preparations can form a stable in-water oil-type emulsion. The lotion formula is optimized then produces the optimal formula obtained on the use of Tween 80 (6.9%) and Span 80 (3.1%). The optimal formula has a pH of 5.78; viscosity of 20613.33 cPs; adhesion of 4.6 seconds; a spread of 6.23 cm/sec; and determination of an SPF value of 17.262. The optimal formula of the optimization method has insignificant differences. The stability test results of the optimum lotion formula show a stable formula result.

FORMULASI LOTION TABIR SURYA EKSTRAK KULIT PISANG Goroho (Musa acuminafe sp) MENGGUNAKAN KONSENTRASI TWEEN 80 DAN SPAN 80

Audia Triani Olii, Mumtihanah Mursyid, Sukma Meilinda Mokodompit — Thu, 31 Oct 2024 00:00:00 +0000

Flavanoids are compounds that have activity as sunscreen. This compound is contained in banana peels and can be used as sunscreen ingredients. The purpose of this research is to optimize the preparation of goroho banana peel extract lotion (Musa acuminafe sp). The use of certain combinations of Tween 80 and Span 80 in lotion preparations has been reported to be able to form an optimal in-water oil-type emulsion. Formula optimization with various variations of Tween 80 and Span 80 concentrations using Design expert version 10 of the Simplex Lattice Design model with pH parameters, viscosity, adhesion, spreadability and SPF determination. The optimal lotion formula was obtained at the use of Tween 80 4.5% and Span 80 5.5%. The optimal formula has a pH of 5.46, a viscosity of 33226.66 dPas, a adhesion of 6.01 seconds, a spread of 6.25 cm/s, and an SPF test and has good asceptability to respondents. The optimal formula of software prediction results with research results shows insignificant differences. This shows that the Simplex Lattice Design method can be used to predict the physical properties parameters of goroho banana peel extract lotion preparations.

level comparison Comparison of Phenolic Content of Rubber Kebo (Ficus elastica) Leaf Extract Results from Multilevel Extraction Using the UV-Vis Spectrophotometry Method

zhafira anggraini mondo, Asriani Suhaenah, Masdiana Tahir — Thu, 31 Oct 2024 00:00:00 +0000

ZHAFIRA ANGGRAINI MONDO. Comparison of Phenolic Content of Rubber Kebo (Ficus elastica) Leaf Extract Results from Multilevel Extraction Using the UV-Vis Spectrophotometry Method (Supervised by Asriani Suhaenah and Masdiana Tahir).

The kebo rubber plant (Ficus elastica) is used by Indonesian people as traditional medicine, namely medicine to lower blood pressure, lower cholesterol, stroke, and reduce joint pain. Kebo rubber leaves (Ficus elastica) contain secondary metabolites such as saponins, polyphenols, flavonoids and tannins. This research aims to determine the comparison of phenolic compound levels of kebo rubber leaf extract (Ficus elastica) resulting from multilevel extraction using the UV-vis spectrophotometric method. The extraction process uses 3 different types of solvents, namely n-hexane (nonpolar), ethyl acetate (semipolar) and ethanol (polar) using a sonicator. Determination of phenolic content using the UV-Vis spectrophotometric method, with a maximum wavelength of 772 nm and using gallic acid as a comparison. The results showed that the levels were influenced by the solvent used in comparing the phenolic compound levels of kebo rubber leaf extract (Ficus elastica) with the multilevel extraction method, namely n-hexane extract 3,774 mgGAE/g, ethyl acetate extract 12,696 mgGAE/g, ethanol extract 74,396 mgGAE/g g.

DETERMINATION OF TANNIN DETERMINATION OF TANNIN CONTENT IN ETHANOL EXTRACT OF KEBO RUBBER LEAVES (Ficus elastica Roxb. ex Hornem) USING UV-VIS SPECTROPHOTOMETRY METHOD

aulia azahra renhoran, Asriani Suhaenah, A. Muflihunna — Thu, 31 Oct 2024 00:00:00 +0000

This research aims to determine the tannin content in the ethanol extract of kebo rubber leaves (Ficus elastica Roxb. ex Hornem). Rubber plants (Ficus elastica Roxb. ex Hornem) are plants that are widely used as traditional medicine, the leaves of this plant are used as medicine to treat diarrhea. , stops bleeding and treats hemorrhoids. Many of the benefits and properties of the kebo rubber plant (Ficus elastica Roxb. ex Hornem) cannot be separated from the compounds contained in it, such as phenolics, tannins, flavonoids and saponins. Simplicia was extracted using the maceration method with 96% concentration of ethanol solvent for 3x24 hours. Determination of tannin content using the UV-Vis spectrophotometric method, with a maximum wavelength of 772 nm, using gallic acid as a comparison. The research results showed that the total tannin content in the ethanol extract was an average of 31.708mgQE/g.

Comparasion Of Levels Comparison of Flavonoid Content of Rubber Kebo (Ficus elastica) Leaf Extract Results of Multilevel Extraction Using UV-Vis Spectrophotometry Method.

nur evatiara, Asriani Suhaenah, Masdiana Tahir — Thu, 31 Oct 2024 00:00:00 +0000

NUR EVATIARA. Comparison of flavonoid levels of rubber plant leaf extract (Ficus elastica) resulting from multilevel extraction using the UV-Vis spectrophotometric method. (Supervised by Asriani Suhaenah and Masdiana Tahir).

Rubber plant (Ficus elastica) is a plant in the Moraceae family whose leaves have been empirically stated to contain secondary metabolite compounds such as saponins, polyphenols, flavonoids and tannins which have been used as medicine for coughing up blood, improving urine flow, relieving pain and improving blood circulation. The aim of this research was to determine the comparison of levels of flavonoid compounds in rubber plant leaf extract (Ficus elastica) resulting from multilevel extraction using the UV-Vis spectrophotometric method. The multistage extraction process uses 3 different types of solvents, namely n-hexane (nonpolar), ethyl acetate (semipolar), and ethanol (polar) using a sonicator. Determination of flavonoid levels using the UV-Vis spectrophotometric method, with a maximum wavelength of 432 nm and quarcetin as a comparison. The research results were influenced by the solvent used in comparing the levels of flavonoid compounds, namely rubber plant leaf extract (Ficus elastica) with a multilevel extraction method, namely N-hexane extract 7.161 mgQE/g, Ethyl acetate extract 7.663 mgQE/g, 96% Ethanol extract 10.592 mgQE/g g.

antioxidant activity Antioxidant activity test of rubber kebo (ficus elastica) leaf extract using multistage maceration method

Indah Ahmad Yani, Asriani Suhaenah, Aminah Aminah — Thu, 31 Oct 2024 00:00:00 +0000

Kebo rubber (Ficus elastica) is one type of plant in Indonesia as a medicine to lower blood pressure, reduce cholesterol, stroke and reduce joint pain. In addition, Kebo rubber (Ficus elastica) leaves are one of the ficus species that are thought to be rich in polyphenolic compounds so that they can act as antioxidants. Antioxidants are compounds that can be used to counteract free radicals. Food fiber and antioxidants are two types of components that are very beneficial in improving health and being able to prevent various diseases. This study aims to analyze the antioxidant activity of rubber kebo (Ficus elastica) leaf extract using the DPPH method. The maceration process uses 3 different types of solvent, namely n-hexane (non-polae), ethyl acetate was measured at 510 nm wavelength with quercetin as the standard comparator. The measurement result for n-hexan extract obtained IC₅₀of 531,90 µg/mL, ethyl acetat obtained IC₅₀of 188,06 µg/mL, and for ethanol extract obtained IC₅₀ of 874,73 µg/mL.

PROFILE OF DRUG STORAGE Profile of medicine storage at the wosu community health center,Morowali Regency

Moh David, Nurlina Nurlina, Iskandar Zulkarnain — Thu, 31 Oct 2024 00:00:00 +0000

Drug storage profiles are an importand factor ini drug management at community health center, because with proper storage of medicines it will be easier and more effective to guarantee the quality of medicines. This research aims to determine the procedures for regulating drugs and improving the quality of drug storage at the wosu healty center, bungku barat sub-district, morowali district. Data collection was carried out through interviews and using observation sheets regarding aspects of drug storage in community health centers including general aspects, special aspectsinclude requirements for drug storage warehouses with 15 aspects assesed with a persentage of 100%, special aspects include requirements of drug preparation with 5 aspects assessed with a percentage of 100% and document review includes procedures for recording drugs. From the result of research in the field there are still several problems that do not meet standards. How ever in general the results of this study show that drug storage, preparation of drugs, and recording of drugs at the wosu comunity health center, bungku barat sub- district, morowali regency are in the good category.

Antioxidant Activity Antioxidant Activity of Ethanol Extract of Lerak Leaves (Sapindus rarak DC) Using DPPH Free Radical Reduction Method

Fitriani Asri, Asriani Suhaenah, St. Maryam — Thu, 31 Oct 2024 02:39:12 +0000

FITRIANI ASRI. Antioxidant Activity Of Ethanol Extract Of Lerak eaves (Sapindus rarak) Using The DPPH Free Radical Reduction Method (Supervised by Asriani Suhaenah dan St. Maryam)

Lerak leaves (Sapindus rarak) are a traditional plant used by the community to treat various diseases. The secondary metabolic compounds contained in the lerak plant (Sapindus rarak) which have potential as antioxidants are flavonoids, alkaloids, saponins, tannins, quinones, steroids and triterpenoids which are obtained through extraction using ethanol solvent. This research aims to determine the antioxidant activity of the ethanol extract of lerak leaves (Sapindus rarak) using the DPPH free radical reduction method (1,1-diphenyl-2-picrihydrazyl). The antioxidant value was measured using UV-Vis spectrophotometry at a wavelength of 516 nm. The calculation results show an IC50 value of 876,592 μg/mL (very weak category) while quercetin as a comparison has very strong antioxidant activity with an IC50 value of 1,882 μg/mL.

Analysis of Chemical Content and Determination of Polyphenol Content of Lerak Leaves (Sapindus rarak DC) Using UV-Vis Spectrophotometry Method

Medina Salim, St. Maryam, A. Muflihunna — Thu, 31 Oct 2024 00:00:00 +0000

MEDINA SALIM. Chemical Content Analysis and Determination of Lerak Leaf Polyphenol Content (Sapindus rarak DC) by UV-Vis Spectrophotometry Method (Guided by St. Maryam and A. Muflihunna). Lerak contains polyphenolic compounds that reduce blood sugar levels. This study aims to determine the chemical content contained in lerak leaf extract (Sapindus rarak DC) and find out how much polyphenol compounds in the sample. The samples were macerated for 3 days using 96% ethanol solvent, the extraction process was carried out until obtaining an extract yield of 9.323 grams, then two tests were carried out, namely qualitative and quantitative, The qualitative test uses a wet reagent while the quantitative test uses the UV-Vis spectrophotometry method. The maximum wavelength used was 768 nm with gallic acid as a comparison. Based on the study, the results of ethanol extract of lerak leaves (Sapindus rarak DC) contain secondary metabolites, namely saponins, steroids, polyphenols and tannins and the result of polyphenol content is 21,168 mgGAE/g.

Antioxidant activity Antioxidant activity of ethanol extract of bitti leaves (Vitex cofassus Reinw. ex. Blume) using the dpph free radical scavenging method

Dita Wulan Bahari, Asriani Suhaenah, A. Muflihunna — Thu, 31 Oct 2024 00:00:00 +0000

Antioxidants are compounds that can counteract free radicals in the body, reduce oxidation in cells and cell damage. The purpose of this study was to determine the antioxidant activity of ethanol extract of bitti leaves (Vitex cofassus Reinw. Ex. Blume with DPPH (2,2- diphenyl-1-picrylhydrazyl) scavenging method. The absorbance was measured by UV-Vis spectrophotometer at 510 nm wavelength. The results showed the presence of antioxidant activity with IC₅₀ value of 56.59 μg/mL with strong antioxidant activity category, while quercetin as a comparator has very strong antioxidant activity with IC50 value of 5.93 μg/mL.

content analysis analysis of Tannin Compound Levels in Tea Leaves of Cocoa Plants (Theobroma cacao L.) Using UV-Vis Spectrophotometry.

nurrizky afiah arifin, Asriani Suhaenah, St. Maryam — Thu, 31 Oct 2024 00:00:00 +0000

Cocoa leaves (Theobroma cacao L.) contain secondary metabolite compounds, namely flavonoids, saponins, tannins, theobromine, caffeine, anthocyanins, leucoanthocyanins and catechol. This research aims to determine the levels of tannin compounds contained in tea preparations from the leaves of the cocoa plant (Theobroma cacao L.) based on a comparison of growing places, namely Wajo, Jeneponto and Malino. The research was carried out qualitatively using wet reagents and quantitatively using the UV-Vis spectrophotometric method. Based on the qualitative test results, FeCl3 1% reagent produces a green solution blackish, the gelatin test produces precipitation, while the potassium ferricyanide and ammonia reagent produces a dark brown color, these results indicate that cocoa leaves contain tannin compounds, for the quantitative test it is measured using UVVis spectrophotometry at a maximum wavelength of 772 nm with a reference, namely gallic acid. . The results obtained for the Wajo area were 1,618mgGAE/g, Jeneponto 2,334mgGAE/g, and Malino 1,030mgGAE/g, based on these data it can be concluded that cocoa leaves contain tannin compounds and the highest levels are in the Jeneponto area.

Activity Test Ethanol Extract Kenikir Leaves (Cosmos caudatus Kunth.) by Using Phosphomolybdate Method

Zainal Abidin, Rais Razak, Bayu Fajar Juniarto — Thu, 31 Oct 2024 00:00:00 +0000

Kenikir leaves (Cosmos caudatus Kunth.) are one of the vegetables that are often consumed as fresh vegetables. Apart from being used as a vegetable, kenikir is also useful as a medicinal plant, including strengthening bones and improving blood circulation. Kenikir leaves contain alkaloids, saponins, steroids, phenols, terpenoids and flavonoids. One of the methods used in the extraction process is the maceration method using 96% ethanol solvent, after which a liquid extract is obtained and then heated using a rotary evaporator to obtain a thick extract. The antioxidant activity test using the phosphomolybdate method is based on the reduction power of phosphomolybdate and the total antioxidant power of the sample. This study aims to determine the antioxidant activity of ethanol extract of kenikir leaves (Cosmos caudatus Kunth.) using the phosphomolybdate method. Determination of antioxidant activity values using a UV-Vis spectrophotometer at a maximum wavelength of 423 nm. The results showed that the standard quercetin solution had an RC50 value of 91.33 μg/mL and the ethanol extract of kenikir leaves (Cosmos caudatus Kunth.) had an RC50 value of 513.49 μg/mL.

Uji Aktivitas Ekstrak Etanol Daun Kenikir (Cosmos caudatus Kunth.) Dengan Metode DPPH

Zainal Abidin, Aminah Aminah, Nurmala Sari — Thu, 31 Oct 2024 07:32:33 +0000

Kenikir (Cosmos caudatus Kunth.) is a traditional vegetable that can be eaten raw or cooked as a vegetable. This plant is also used for food flavoring and traditional medicine. In addition, several nutritional and medical studies show that Kenikir is rich in bioactive compounds such as flavonoids, phenols, carbohydrates, minerals, proteins and vitamins, which can increase its nutritional value. This study aims to determine the antioxidant activity of ethanol extract of kenikir leaves (Cosmos caudatus Kunth) using the DPPH method. The results showed that the ethanol extract of kenikir leaves (C. caudatus Kunth) had high antioxidant activity. Antioxidants are substances that can delay, slow down and prevent oxidation. Antioxidants are very beneficial for health and play an important role in maintaining the quality of food products. The antioxidant activity is in the very strong category (<50 ppm), namely 25,212 ppm. The macerated extract showed the highest antioxidant activity because it contained many secondary metabolites such as flavonoids and phenolics.