Antioxidant Activity Test of Pulutan (Urena lobata Linn) Leaf Ethanol Extract from Barru District Using the DPPH Method
Abstract
Pulutan (Urena lobata Linn) is one of the many wild plants that can be found in Barru district. Pulutan leaf extract has several properties including being an antioxidant, antibacterial, antifungal, and can inhibit the growth of breast cancer cells. Pulutan leaf extract contains flavonoids, alkaloids, cardiaglycosides, tannins, terpenoids and saponins. This study aims to analyze the antioxidant activity of the ethanol extract of pulutan leaves using the DPPH free radical scavenging method. This type of research is laboratory experimental using the spectrophotometer method based on the calculation of the IC50 value of the extract on DPPH free radical activity.The stages of this research began with the preparation and extraction of samples, preparation of blank solutions and measurement of the maximum wavelength, and ended with testing the antioxidant activity of extracts and quercetin standards. This research obtained yields of percent reduction of 3.241%; the value of the extract regression equation is the value of y = 0.0693x + 30.489 (r = 0.9963); and IC50 value of 281 μg/mL. The results of this study illustrate that the ethanol extract of pulutan leaves has antioxidant activity but is in a weak antioxidant category.
Keywords: Pulutan (Urena lobata Linn) leaves; antioxidants; DPPH; Spektrofometer uv-vis
References
[2] Nurhasanah, T. et al. (2019) „Karakterisasi Simplisia dan Ekstrak Serta Identifikasi Histokimia Daun Pulutan ( Urena Lobata L .) Crude Drugs Extract Characterization and Histochemical Identification of Pulutan Leaves‟, Prosiding Farmasi, pp. 28–35.
[3] Fadillah, U.F. and Hambali, Identifikasi Senyawa Aktif Ekstrak Daun Pulutan (Urena lobata L) dengan GC-MS 2019. „Jurnal Sains dan Kesehatan‟, 2(3).
[4] Handajani, F. (2019) Oksidan dan Antioksidan Pada Beberapa Penyakit dan Proses Penuaan. sidoardjo: zifatama jawara.
[5] Fakriah. et al. (2019) ‘Sosialisasi Bahaya Radikal Bebas Dan Fungsi Antioksidan Alami Bagi Kesehatan‟, Jurnal Vokasi, 3(1), p. 1.
[6] Kurniawati, D. (2021) Potensiformulasi Infusa Daun Sirih (Piper betle L) Ekstrak Kulit Jeruk Nipis (Citrus aurantifolia) dan Ekstrak Bundung ( Actinoscirpus grossus) Sebagai Terapi Kandidiasis. Penerbit NEM.
[7] Megawati et al.2019. Uji Aktivitas Antioksidan Ekstrak Etanol Daun Murbei (Morus alba L) Asal Kupang, Nusa Tenggara Timur Dengan Metode DPPH (2,2 DIPHENIL-1- PICRYLHYDRAZYL),Jurnal Penelitian Volume 08 Nomor 01 Mei 2019
[8] Handayani, Virsa et al.2014. Uji Aktivitas Antioksidan Ekstrak Metanol Bunga dan Daun Patikala ( Etlingera alatior (Jack) R.M.Sm) Menggunakan Metode DPPH. Pharm Sci Res ISSN 2407-2354
[9] Hasnaeni, Widdawati, Suriati Usman. 2019. Pengaruh Metode Ekstraksi Terhadap Rendamen Dan Kadar Fenolik Ekstrak Tanaman Kayu Beta-Beta (Lunasia amara Blanco).Jurnal Farmasi Galenika
[10] Febria,Whika.2020. Perbandingan Pelarut Kloroform dan Etanol terhadap Rendemen Ekstrak Daun Lidah Mertua (Sansevieria trifasciata Prain.) Menggunakan Metode Maserasi.Journal Uin Alaudin
[11] Adawiyah,Rabiatul Et al.2018. Aktivitas Antioksidan Ekstrak Etanol Akar Kalakai (Stenochlaena palustris Bedd) Asal Kalimantan Tengah. Jurnal Pharmascience, Vol. 05 , No.01
[12] Nia,Rakhmadhan and Helda.2016. Aktivitas Antioksidan Ekstrak Etanol Kulit Buah Naga Merah Daerah Pelaihari, Kalimantan Selatan Dengan Metode DPPH (2,2-difenil-1-pikrilhidrazil). Jurnal Pharmascience, Vol .03, No.02, Oktober 2016, hal: 36 - 42
