Antioxidant Activity Test of Ketapang Leaf Extract (Terminalia catappa L.) Using the B-Carotene Bleaching Method
Abstract
Ketapang (Terminalia catappa L.) is a plant that belongs to the Combretaceae family which is spread in tropical countries, including Indonesia. Ketapang leaves (Terminalia catappa L.) contains flavonoid compounds which function as antioxidants. The purpose of this study was to determine the antioxidant activity of ketapang leaf extract (Terminalia catappa L.) using the β-carotene bleaching method. The extract was obtained through the maceration method, then the yield percentage was 20.25%. Phytochemical screening test was carried out identifiy chemical compounds and obtained namely alkaloids, flavanoids, tannins, saponins, and steroids. Antioxidant activity was performed using UV-Vis spectrophotometry with a wavelength of 538 nm. The results showed that the antioxidant activity of ketapang leaf extract (Terminalia catappa L.) of 56.5% was an intermediate antioxidant (moderate), then in a standard solution of quercetin has a strong antioxidant activity of 72.3%.
Keywords : Ketapang leaves (Terminalia catappa L.), Screening Phytochemicals, Antioxidants, B-carotene bleaching, Spectrophotometry UV-Vis.
References
[2] Adrianta Agus Ketut, (2020). "Program Studi Sarjana Farmasi, Fakultas Farmasi Universitas Mahasaraswati Denpasar Jl. Kamboja No. 11 A, Denpasar Bali" American Journal of Tropical Medicine and Hygiene, 6 (1), 33–39.
[3] Salimi. K. Yuszda : “Daun Miana Sebagai Antioksidan Dan Antikanker”; Yayasan Pendidikan dan Sosial Indonesia Maju (YPSIM) Banten, 2021
[4] Salimi, Y. K., Kamarudin, J., Ischak, N. I., & Bialangi, N. (2022). "Aktivitas Antioksidan Senyawa Metabolit Sekunder Ekstrak Metanol Daun Ketapang (Terminalia catappa L.)". 4 (2), 12–21.
[5] Abdulkadir Rabiu Abdulaziz. (2015). “ In Vitro Antioxidant Activity of Ethanolic Extract from Terminalia Catappa (L.) Leaves and Fruits: Effect of Fruit Ripening” International Journal of Science and Research (IJSR). Vol 4, No 8. ISSN : 2319-7064
[6] Kementerian Kesehatan RI. 2017. Farmakope Herbal Indonesia Edisi II. Jakarta: Kementerian Kesehatan RI.
[7] Do et el, 2014), Uji Aktivitas Antioksidan Fraksi Etil Asetat Limbah Daun Ketapang (Terminalia Catappa L.) Menggunakan Metode Dpph (1,1-Difenil-2pikrilhidrazin) Universitas Islam Negeri Walisongo Semarang.
[8] Mangela Oktavian, Ridhay Ahmad, Musafira. Kajian Aktivitas Antioksidan Ekstrak Daun Tembelekan (Lantana camara L). Berdasarkan Tingkat Kepolaran Pelarut. Kovalen, 2(3) : 16-23, Desember 2016 NO ISSN: 2477-5398.
[9] Kumoro, A.C. 2015. Teknologi Ekstraksi Senyawa Bahan Aktif dari Tanaman Obat. Yogyakarta: Plantaxia.
[10] Rossalinda, Wijayanti, F., & Iskandar, D. (2021). 'Effectiveness Of Matoa Leaf (Pometia Pinnata) Extract As An Antibacterial Staphylococcus Epidermidis'. Stannum : Jurnal Sains Dan Terapan Kimia, 3(1), 1–8.
[11] Salamah, N., dan Nurushoimah, 2014, “Uji Aktivitas Antioksidan Ekstrak Etanol Herba Pegagan (Centella asiatica (L.) Urb.) dengan Menggunakan Metode Beta Karoten”, Jurnal Farmasi.
[12] Wahyulianingsih, Handayani, S., & Malik, A. (2016). 'Penetapan Kadar Flavonoid Total Ekstrak Daun Cengkeh'. Jurnal Fitofarmaka Indonesia, 3(2).
[13] Yennie, E., S. Elystia, A. Kalvin dan M. Irfhan, 2013, “Pembuatan pestisida organik menggunakan metode ekstraksi dari daun pepaya dan umbi bawang putih”. Jurnal Teknik Lingkungan Unand, 10 (1) : 46-59.
[14] Wendersteyt, N. V., Wewengkang, D. S., & Abdullah, S. S. (2021). 'Uji Aktivitas Antimikroba dari Ekstrak dan Fraksi Ascidian Herdmania momus dari Perairan Pulau Bangka Likupang Terhadap Pertumbuhan Candida albicans'. Pharmacon, 10(1), 706.
[15] Toar Waraney Senduk , Lita A. D. Y. Montolalu , Verly Dotulong. 2020. Rendemen Ekstrak Air Rebusan Daun Tua Mangrove Sonneratia Alba. Jurnal Perikanan dan Kelautan Tropis ,Vol 11 no 1, e_ISSN :2302-6081 p_ISSN 2302-609X
[16] Astarina, N. W. G., Astuti, K. W., & Warditiani, N. K. (2013). 'Skrining Fitokimia Ekstrak Metanol Rimpang Bangle (Zingiber purpureum Roxb.)'. Jurnal Farmasi Udayana, 12(2009), 793–794.
[17] Shetty, K., Paliyath, G., Pometto, A., dan Levin, R, E., (2017), Functional Foods and Biotechnology, CRC. Press, USA.
[18] Hasan. N. M., (2015). Uji Kandungan Flavonoid dan Perbandingan Aktivitas Antioksidan Pada Ekstrak Etanol Simplisia Bunga Pepaya Gantung Saat Kuncup dan Mekar. Biologi Saintek UIN Maliki Malang.